Submit Manuscript  

Article Details


Swimming Impacts on Pancreatic Inflammatory Cytokines, miR-146a and NF-кB Expression Levels in Type-2 Diabetic Rats

Author(s):

Mohammad Reza Alipour, Nasibeh Yousefzade, Fariba Mirzaei Bavil, Roya Naderi and Rafighe Ghiasi*   Pages 1 - 6 ( 6 )

Abstract:


Background: Obesity-induced chronic inflammation is a key component in the pathogenesis of insulin resistance and type-2 diabetes

Objective: This study aimed to evaluate the effect of swimming exercise on pancreatic expression levels of inflammatory cytokines, miR-146a and NF-кB in type-2 diabetic male rats.

Method: Twenty- eight male Wistar rats were divided into four groups: control (Con), exercise, diabetes and diabetic exercise (n = 7). Diabetes induction performed by the combination of high-fat diet (HFD, 4 weeks) and streptozotocin (35 mg/kg. ip). After induction of diabetes, the rats swam in the exercise groups for 12 weeks. Then, blood and tissue samples were collected.

Result: Our results indicated a significant increase in expression levels of miR-146, NF-κB and inflammatory cytokines (IL-6, TNF-α, and IL-1β) while a significant decrease in pancreatic expression levels of TRAF6 and IRAK1 in diabetic group as compared to the control group. In contrast, swimming exercise resulted in a significant decrease in expression levels of miR-146a, NF-кB and inflammatory cytokines and a significant increase in expression levels of TRAF6 and IRAK1 in the exercise-diabetic group compared to the diabetic group.

Conclusion: Our results indicated a significant increase in expression levels of miR-146, NF-κB and inflammatory cytokines (IL-6, TNF-α, and IL-1β) while a significant decrease in pancreatic expression levels of TRAF6 and IRAK1 in diabetic group as compared to the control group. In contrast, swimming exercise resulted in a significant decrease in expression levels of miR-146a, NF-кB and inflammatory cytokines and a significant increase in expression levels of TRAF6 and IRAK1 in the exercise-diabetic group compared to the diabetic group.

Keywords:

Diabetes, inflammatory cytokines, NF-κB; miR-146a, IRAK1, TRAF6

Affiliation:

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Department of Physiology, Tabriz Faculty of Medical Science Tabriz University of Medical Sciences, Tabriz, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Department of Physiology, Urmia Faculty of Medical Science, Nephrology and Kidney Transplant Research Center, Urmia University of Medical Science, Urmia, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz



Read Full-Text article