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Methylation Profile of miR-9-1 and miR-9-1/-9-3 as Potential Biomarkers of Diabetic Retinopathy

[ Vol. 17 , Issue. 6 ]

Author(s):

Caroline Severo de Assis*, Alexandre Sérgio Silva, Mayara Karla dos Santos Nunes, João Modesto Filho, Rayner Anderson Ferreira do Nascimento, Cecília Neta Alves Pegado Gomes, Isabella Wanderley de Queiroga Evangelista, Naila Francis Paulo de Oliveira and Darlene Camati Persuhn   Pages 8 - 16 ( 9 )

Abstract:


Aims: Analysis of the relationship between the methylation profile of miR-9-1 or miRs -9-1 / -9-3 and diabetic retinopathy.

Background: Diabetic Retinopathy (DR) is a frequent complication of Diabetes mellitus and it has a decisive impact on the quality of life, as it is one of the biggest causes of blindness in the adult population. Levels of microRNA-9 have been shown to be related to diabetes but little is known about its involvement with DR in humans.

Objective: To analyze the relationship between the methylation profile of miR-9-1 or miRs -9-1/-9-3 and DR.

Methods: 103 patients diagnosed with diabetes for 5 to 10 years were analyzed. The data were categorized according to clinical, biochemical, lifestyle and anthropometric parameters. DNA extracted from leukocyte samples was used to determine the methylation profile of miRs-9-1 and -9-3 using a specific methylation PCR assay.

Results: miR-9-1 methylation was related to diabetic retinopathy, indicating that methylation of this miR increases the chances of presenting retinopathy up to 5 times. In our analyses, diabetics with lower levels of creatinine and CRP showed significant reductions (99% and 97%) in presenting DR. Methylation of both miRs-9-1 and 9-3 methylated increases the chances of presenting DR by 8 times; in addition, a sedentary lifestyle can increase the risk for the same complication by up to 6 times.

Conclusion: Our results suggest that both methylation of miR-9-1 and e miRs-9-1 / 9-3 favors DR in patients with diabetes in a period of 5 to 10 years of diagnosis.

Keywords:

Diabetes, diabetic retinopathy, microRNAs, methylation, diabetes time, sedentary lifestyle.

Affiliation:

Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Department of Physical Education, Federal University of Paraiba, Joao Pessoa, Post-Graduation Program in Development and Technological Innovation of Medicines (DITM), Federal University of Paraiba, Joao Pessoa, Department of Internal Medicine, Federal University of Paraiba, Joao Pessoa, Uninassau Faculty, Federal University of Paraiba, Joao Pessoa, Nephrology Clinic, LauroWanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Ophthalmology Reference Center, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa, Department of Molecular Biology and Post-Graduation Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa



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